Research Article
Year 2024,
Volume: 7 Issue: 1, 1 - 6, 01.01.2024
Abstract
Nowadays, there has been a growing interest in finding alternative protein sources for both the food industry and nutritional purposes. Protein experts have recently focused on investigating watermelon seeds, which are not only a food processing waste but also contain high-quality proteins. Therefore, this study aimed to achieve maximum protein extraction from watermelon seeds using an ultrasound-assisted extraction process. The study investigated the effects of pH (A; 7─11), sonication temperature (B; 30─60 °C), and sonication time (C; 5─15 min) on protein recovery to develop a Taguchi model. Through optimization, the optimal conditions for maximum protein recovery (85.81%) within the range of process variables were found to be 11 pH, 45 °C sonication temperature, and 10 min sonication time (A3B2C2). An analysis of variance (ANOVA) revealed that pH and sonication temperature significantly influenced the protein extraction process (P<0.05). The optimized extraction conditions resulted in a remarkable improvement (56.79%) in protein recovery compared to the initial process parameters (A1B1C1). This study demonstrates the effectiveness of the proposed extraction model for obtaining proteins from high-protein seed sources.
Keywords
References
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- Gadalkar SM, Rathod VK. 2020. Extraction of watermelon seed proteins with enhanced functional properties using ultrasound. Preparat Biochem Biotechnol, 50(2): 133-140.
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- Guldane M, Dogan M. 2022. Multi-response optimization of process parameters of saponin-based model foam using Taguchi method and gray relational analysis coupled with principal component analysis. J Food Proc Preservat, 46(5): 1-14.
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- Liu L, Xi J. 2021. Mechanochemical-assisted extraction of protein from watermelon seeds with surfactant. Lwt, 142: 111025.
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- Pathak U, Kumari S, Kumar A, Mandal T. 2020. Process parametric optimization toward augmentation of silica yield using Taguchi technique and artificial neural network approach. Energy Ecol Environ, 5(4): 294-312.
- Qin D, Wang Y, Wu Y, Kong X, Liu L, Li Z, Xi J. 2021. Optimization of protein extraction from watermelon seeds by liquid-phase pulsed discharge based on energy input for scale-up application. Lwt, 152: 112355.
- Wani AA, Kaur D, Ahmed I, Sogi DS. 2008. Extraction optimization of watermelon seed protein using response surface methodology. Lwt, 41(8): 1514-1520.
- Wani AA, Sogi DS, Singh P, Shivhare US. 2011. Characterization and functional properties of watermelon (Citrullus lanatus) seed protein isolates and salt assisted protein concentrates. Food Sci Biotechnol, 20(4): 877-887.
- Wani AA, Sogi DS, Singh P, Wani IA, Shivhare US. 2011. Characterisation and functional properties of watermelon (Citrullus lanatus) seed proteins. J Sci Food Agri, 91(1): 113-121.
Year 2024,
Volume: 7 Issue: 1, 1 - 6, 01.01.2024
Abstract
References
- Abas Wani A, Sogi DS, Grover L, Saxena DC. 2006. Effect of temperature, alkali concentration, mixing time and meal/solvent ratio on the extraction of watermelon seed proteins-a response surface approach. Biosyst Eng, 94(1): 67-73.
- Ayoubi-Feiz B, Soleimani D, Sheydaei M. 2019. Taguchi method for optimization of immobilized Dy2O3/graphite/TiO2/Ti nanocomposite preparation and application in visible light photoelectrocatalysis process. J Electroanalyt Chem, 849: 113377.
- Behere M, Patil SS, Rathod VK. 2021. Rapid extraction of watermelon seed proteins using microwave and its functional properties. Prepar Biochem Biotechnol, 51(3): 252-259.
- Biswas B, Sit N. 2020. Effect of ultrasonication on functional properties of tamarind seed protein isolates. J Food Sci Technol, 57(6): 2070-2078.
- Bose PK, Deb M, Banerjee R, Majumder A. 2013. Multi objective optimization of performance parameters of a single cylinder diesel engine running with hydrogen using a Taguchi-fuzzy based approach. Energy, 63: 375-386.
- Dimou M, Marnasidis S, Antoniadou I, Pliatsika M, Besseris GJ. 2009. The application of Taguchi method to determine the optimum blend of unifloral honeys to most closely match thyme honey quality. Int J Food Sci Technol, 44(10): 1877-1886.
- Fatima K, Imran M, Ahmad MH, Khan MK, Khalid W, AL-Farga A, Alansari WS, Shamlan G, Eskandrani AA. 2023. Ultrasound-assisted extraction of protein from moringa oleifera seeds and ıts ımpact on techno-functional properties. Molecules, 28(6): 2554.
- Firatligil-Durmus E, Evranuz O. 2010. Response surface methodology for protein extraction optimization of red pepper seed (Capsicum frutescens). Lwt, 43(2): 226-231.
- Gadalkar SM, Rathod VK. 2020. Extraction of watermelon seed proteins with enhanced functional properties using ultrasound. Preparat Biochem Biotechnol, 50(2): 133-140.
- Güldane M. 2023. Optimizing foam quality characteristics of model food using Taguchi-based fuzzy logic method. J Food Proc Eng, 46(8): e14384.
- Guldane M, Dogan M. 2022. Multi-response optimization of process parameters of saponin-based model foam using Taguchi method and gray relational analysis coupled with principal component analysis. J Food Proc Preservat, 46(5): 1-14.
- Güldane M, Doğan M. 2020. The effect of process parameters on color and density properties of foam halva: Taguchi mathemetical model optimization. J Food, 45(6): 1248-1260.
- Kannan G, Thangaraju R. 2022. Effect of industrial waste fly ash on the drilling characteristics of banana fiber residue reinforced polymer composites. J Indust Textiles, 51(2): 2665S-2687S.
- Liu L, Xi J. 2021. Mechanochemical-assisted extraction of protein from watermelon seeds with surfactant. Lwt, 142: 111025.
- Liu RL, Yu P, Ge XL, Bai XF, Li XQ, Fu Q. 2017. Establishment of an aqueous peg 200-based deep eutectic solvent extraction and enrichment method for pumpkin (Cucurbita moschata) seed protein. Food Analyt Methods, 10(6): 1669-1680.
- Pathak U, Kumari S, Kumar A, Mandal T. 2020. Process parametric optimization toward augmentation of silica yield using Taguchi technique and artificial neural network approach. Energy Ecol Environ, 5(4): 294-312.
- Qin D, Wang Y, Wu Y, Kong X, Liu L, Li Z, Xi J. 2021. Optimization of protein extraction from watermelon seeds by liquid-phase pulsed discharge based on energy input for scale-up application. Lwt, 152: 112355.
- Wani AA, Kaur D, Ahmed I, Sogi DS. 2008. Extraction optimization of watermelon seed protein using response surface methodology. Lwt, 41(8): 1514-1520.
- Wani AA, Sogi DS, Singh P, Shivhare US. 2011. Characterization and functional properties of watermelon (Citrullus lanatus) seed protein isolates and salt assisted protein concentrates. Food Sci Biotechnol, 20(4): 877-887.
- Wani AA, Sogi DS, Singh P, Wani IA, Shivhare US. 2011. Characterisation and functional properties of watermelon (Citrullus lanatus) seed proteins. J Sci Food Agri, 91(1): 113-121.
There are 20 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Food Engineering, Chemical Engineering (Other), Agricultural Engineering (Other) |
| Journal Section | Research Articles |
| Authors | |
| Early Pub Date | November 26, 2023 |
| Publication Date | January 1, 2024 |
| Submission Date | September 1, 2023 |
| Acceptance Date | November 7, 2023 |
| Published in Issue | Year 2024 Volume: 7 Issue: 1 |
